Table 2.

The change in ΔE of a mutant pigment relative to its ancestral pigment (δE) and individual contributions δE_113+114+118 (d₁), δE_90+W1 (d₂), and δE_86+W2 (d₃) in kcal·mol⁻¹

Pigment	Mutation	δE	δE113+114+118(d1)	δE90+W1(d2)	δE86+W1(d3)
a	Phe86 deletion	−0.4	4.4	−15.8	10.6
b	Phe86 deletion	−9.4	0.6	−18.3	9.1
b-aa	Phe86 deletion	−9.0	−3.8	−2.5	−1.5
d	d-7m	−11.2	−11.3	0.4	0.8
f	f′	−4.1	−4.2	0.9	−1.5
	f′/S86C	−1.3	−3.9	1.2	0.5
	f′/S90C	−0.4	−4.8	5.8	−2.4
	f′/S86C/S90C	−0.7	−4.1	6.3	−3.3
f′	S86C	2.8	0.3	0.3	2.0
	S90C	3.9	−0.6	4.9	−0.9
	S86C/S90C	3.4	0.1	5.4	−1.8
h	F86S/L116V	−10.8	−12.8	2.3	−3.1
	F86Y	−10.7	−12.8	2.3	−3.1
	h-7m	−11.4	−11.7	2.5	−3.2

^aThe difference in the first two rows.