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. Author manuscript; available in PMC: 2012 Aug 19.
Published in final edited form as: ACS Chem Biol. 2011 May 24;6(8):808–818. doi: 10.1021/cb200062a

Figure 1. Anisomycin induces JNK-mediated cell death in HeLa cells.

Figure 1

(A) Anisomycin stress induces a JNK dependent cell death. Cell viability was monitored following 4 hours of 25µM anisomycin treatment (grey bars) or 0.1% DMSO (white bars). Cells were either pretreated for 30 minutes prior to anisomycin treatment with 10µM Tat-Scramble peptide or 1µM TI-JIP peptide. Additionally, cell viability was also determined by silencing JNK via siRNA-mediated knockdown. Cells were transfected with 50nM siRNA (control or JNK-specific) 66 hours prior to treatment with 25µM anisomycin. Silencing was monitored by Western blot analysis of 30µg cell lysate following 72 hours of silencing (inset). (B) Anisomycin stress activates JNK signaling. JNK signaling was evaluated by Western blot following 0, 5, 10, 15, 30, 60, and 120 minutes of treatment with 25µM anisomycin. Proteins (30µg) were resolved by SDS-PAGE, and antibodies for Phospho-JNK, JNK, Phospho-c-jun, and c-jun were used to detect proteins of interest. α-Tubulin was used as a loading control.