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. Author manuscript; available in PMC: 2012 Aug 19.
Published in final edited form as: ACS Chem Biol. 2011 May 24;6(8):808–818. doi: 10.1021/cb200062a

Table 1.

Binding and Inhibitory Data for JNK-interacting Peptides for JNK3α1.

Peptide Kd* IC50
-vs-Sab		 IC50
-vs-c-jun		 IC50
-vs-ATF2
TI-JIP 0.45±0.06µM 18±9nM 38±12nM 27±15nM
SabKIM1 11±2.4µM 218±85nM n.i.*** n.i.***
Scramble n.b.** n.i.*** n.i.*** n.i.***
*

Kd was determined using the fluorescent polarization assay described in the methods.

**

No binding was observed for peptide up to 100µM.

***

The peptide demonstrated less than 10% inhibition at a concentration of 10µM. IC50s were determined using a Kinase-Glo assay and the data were fit to a 4-parameter nonlinear regression model.