Figure 3.

DNA-binding assay and cytokine measurements: (a) TransAM NF-κB Family Transcription Factor Assay was used to measure RelA, RelB, and p52 DNA binding following NIKwt and NIKdn overexpression in HK-2 cells. For each Rel protein, ev (left), NIKwt (middle), and NIKdn (right) are shown. Data represent two separate experiments. One-way ANOVA: P < 0.02. (b) Cytokine measurements determined using tissue culture media obtained from the same experiments. Data represent two separate experiments. Two-way ANOVA: P < 0.008. Student's t-test versus empty vector control: *P < 0.05.