Figure 4.

The intracellular ROS production was detected by DCF-DA method. (a) Representative images under fluroscence microscope (original magnification 400x), (b) intensity of DCF fluorescence (indicator of intracellular ROS level) measured using a fluorescence spectrophotometer. Vehicle: ROS level in normal animals, AEPD: ROS level in the animals treated with AEPD only; CCl₄: ROS level in the CCl₄ intoxicated animals; AEPD + CCl₄: ROS level in the animals treated with AEPD prior to CCl₄ intoxication; CCl₄ + AEPD: ROS level in the animals treated with AEPD post-CCl₄ intoxication and Recovery: ROS level in the animals of recovery group. Data are mean ± SD, for six animals per group and were analyzed by one-way ANOVA, with Student-Newman-Keuls post hoc tests. Differences were attributed at P < .05, and homogeneous subgroups share common superscripted letters.