Table 5.
Extraction Efficiency, Matrix Effect and Process Efficiency for Cannabinoids and Cannabinoid Glucuronides in Whole Blood by LC-MS/MS
| Analyte | Extraction efficiency (%, n = 6)
|
Matrix effect (%, n = 6)
|
||||
|---|---|---|---|---|---|---|
| Low | Mid | High | Low | Mid | High | |
| THCa,b | 50.5 | 57.9 | 75.6 | −9.5 | −8.1 | 0.0 |
| 11-hydroxy-THC | 71.5 | 84.6 | 82.3 | −56.4 | −60.1 | −66.6 |
| 11-nor-9-carboxy-THC | 66.8 | 77.1 | 93.9 | −10.5 | −20.0 | −32.1 |
| cannabidiol | 63.2 | 68.5 | 83.8 | −16.8 | −16.1 | −30.1 |
| cannabinol | 56.4 | 63.2 | 76.5 | −19.7 | −12.8 | −3.7 |
| THC-glucuronidec | 73.2 | 75.6 | 87.6 | 48.1 | 41.3 | 1.7 |
| THCCOOH-glucuronided | 56.1 | 58.2 | 57.3 | 8.7 | 9.0 | −10.3 |
| THC-d3 | 50.6 | 57.3 | 71.8 | −11.3 | −8.2 | 6.0 |
| 11-hydroxy-THC-d3 | 79.9 | 84.2 | 79.3 | −57.6 | −59.3 | −65.3 |
| 11-nor-9-carboxy-THC-d9 | 70.9 | 77.6 | 87.3 | −14.1 | −17.8 | −26.5 |
| cannabidiol-d3 | 63.3 | 67.0 | 81.2 | −18.0 | −16.8 | −26.7 |
a
Δ9-tetrahydrocannabinol.
b
THC, 11-hydroxy-THC, 11-nor-9-carboxy-THC, cannabidiol and cannabinol low-, mid- and high-quality control target concentrations were 2.5, 25 and 75 μg/L, respectively.
c
THC-glucuronide low-, mid- and high-quality control target concentrations were 1.5, 15 and 45 μg/L, respectively.
d
THCCOOH-glucuronide low-, mid- and high-quality control target concentrations were 7.5, 75 and 225 μg/L, respectively.