Quantitative PCR verification of gene expression changes observed in the
microarray analyses. A: Technical replicate performed on the original cohort of
animals from microarray studies confirms the microarray results. B: Biological
replicate performed on an additional cohort of animals confirms regulation of
genes of the Wnt signaling pathway. Slit2 (p<=0.01; not shown) and Tgfb3
were also examined to verify findings in the original cohort. Values were
normalized to the control genes beta actin, alpha tubulin, 18s RNA, and GtfIIB.
For a complete list of gene names, please refer to tables 1-3. Statistical
significance was determined with a student’s t-test, * p<=0.05.
Average + SEM; technical replicate vehicle n=6; cocaine n=6; biological
replicate vehicle n=10; cocaine n=8.