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. Author manuscript; available in PMC: 2012 Sep 15.
Published in final edited form as: Biol Psychiatry. 2011 May 14;70(6):583–592. doi: 10.1016/j.biopsych.2011.03.035

Figure 4. Adolescent cocaine exposure affects the expression of synaptic and developmental genes in the amygdala.

Figure 4

Quantitative PCR verification of gene expression changes observed in the microarray analyses. A: Technical replicate performed on the original cohort of animals from microarray studies confirms the microarray results. B: Biological replicate performed on an additional cohort of animals confirms regulation of genes of the Wnt signaling pathway. Slit2 (p<=0.01; not shown) and Tgfb3 were also examined to verify findings in the original cohort. Values were normalized to the control genes beta actin, alpha tubulin, 18s RNA, and GtfIIB. For a complete list of gene names, please refer to tables 1-3. Statistical significance was determined with a student’s t-test, * p<=0.05. Average + SEM; technical replicate vehicle n=6; cocaine n=6; biological replicate vehicle n=10; cocaine n=8.