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. Author manuscript; available in PMC: 2012 Feb 11.
Published in final edited form as: Nature. 2011 May 26;476(7359):220–223. doi: 10.1038/nature10202

Figure 2. NeuroD1 increases reprogramming efficiency in primary human fetal fibroblasts.

Figure 2

a, Quantification of Tuj1-positive BAM-iN cells with indicated factors, 3 weeks after dox. b–c, Three weeks after dox BAM+NeuroD1 iN cells exhibited neuronal morphologies (b) and expressed Tuj1 (c) . d–f, iN cells expressed NeuN (d,) PSA-NCAM (e), and MAP2 (f) 2 weeks after dox. g–h, An iN cell expressing MAP2 (g) and synapsin (h) 4 weeks after dox and co-cultured with primary astrocytes. i, Single cell gene expression profiling using Fluidigm dynamic arrays. Rows represent the evaluated genes and columns represent individual cells. Heatmap (blue to red) represents the threshold Ct values as indicated. Data in (a) are presented as mean±SD. Scale bars: 100 µm (b, c), 10 µm (d–h).