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. 2011 Jul 20;141(9):1754–1761. doi: 10.3945/jn.111.141176

FIGURE 3.

FIGURE 3

 Se in the form of selenoproteins is essential for Arg-I expression in RAW 264.7 macrophage cells and bone marrow-derived macrophage cells. (A) RAW 264.7 cells were cultured with 100 nmol/L methylseleninic acid (MSA) or 100 nmol/L selenomethionine (Se-Met) for 4 d and stimulated with IL-4 (5 mg/L, 20 h). Values are means ± SEM, n = 3. **P < 0.01, ***P < 0.001. All means were compared to one control (Se-deficient diet RAW 264.7 cells) and analyzed using ANOVA with Dunnett’s post hoc testing. (B) Bone marrow-derived macrophage cells isolated from glutathione peroxidase-1 (GPX1) knockout (KO) and glutathione peroxidase-1 wild-type (WT) C57bl/6 mice were cultured with 100 nmol/L sodium selenite for 3 d and stimulated with IL-4 (5 mg/L, 20 h) and LPS (1 mg/L, 12 h). Values are means ± SEM, n = 3. Within each graph, means without a common letter differ, P < 0.01. Se-A, selenium-adequate; Se-D, selenium-deficient.