FIGURE 5.

 STAT6 pathway and hematopoietic PG D₂ synthase inhibitor treatment plays a critical role in the expression of Arg-1 in Se-adequate (Se-A) macrophages. (A) Bone marrow-derived macrophages isolated from Se-deficient (Se-D) and Se-A mice were pretreated with 100 μmol/L leflunomide for 2 h, followed by IL-4 (5 mg/L, 20 h) and LPS (1 mg/L, 12 h). (B) Bone marrow-derived macrophages isolated from Se-deficient and Se-A mice were pretreated with 25 μmol/L HQL-79 for 2 h followed by stimulation with IL-4 (5 mg/L, 20 h) and LPS (1 mg/L, 12 h). Values are means ± SEM, n = 3. Within each graph, means without a common letter differ, P < 0.01.