Figure 4.

(revised from [18]) Limited therapeutic time windows for post-ischemic moderate hypothermia in a focal ischemia with complete reperfusion. (a) A diagram for experimental procedures comparing the protection of hypothermia. Rats were divided into 4 groups. Group 1, normothermia: body temperature was maintained at 37°C throughout the experiment. Group 2, intraischemic hypothermia: hypothermia was induced at ischemic onset and maintained for 3 h. Group 3, early hypothermia: body temperature was adjusted to 30°C 15 min before reperfusion and maintained for 3 h. Group 4, delayed hypothermia: body temperature was adjusted to 30°C 15 min after reperfusion and maintained for 3 h. (b) The upper panel shows representative infarcts stained by TTC. White areas are the infarct regions. The lower panel shows quantitation of infarct volumes. Values are mean ± S.E.M. (n = 8 per each group). ***P < 0.0001, versus normothermia.