Table 1.
Temporal pattern of thoracic luciferase expression.
| Vehicle | Viral load (viral particles) | Days after instillation | |||
|---|---|---|---|---|---|
| 1 | 2 | 4 | 7 | ||
| PP2:PP9 | 5 × 1010 | 4,021 ± 950 | 1,127 ± 275 | 822 ± 100 | 227 ± 37 |
| Saline | 5 × 1010 | 460 ± 665 | 2,246 ± 2,787 | 707 ± 675 | 453 ± 185 |
| PP2:PP9 | 1 × 1010 | 988 ± 1,237 | 278 ± 228 | 313 ± 213 | 283 ± 299 |
| Saline | 1 × 1010 | 232 ± 167 | 79 ± 63 | 194 ± 43 | 260 ± 60 |
Animals were instilled with either 5 × 1010 viral particles (n = 3/group) or 1 × 1010 viral particles (n = 4/group) rAd-Luc intratracheally using either saline or as a PP2:PP9 PFC suspension. Mice were anesthetized, and images were acquired at the time indicated using a cooled CCD camera and imaging system (IVIS50, Xenogen Corp). Luminescence was quantified using Living Image software with a constant region of interest. Values represent the mean ± standard deviation.