Table 1.

Melting temperatures for the three aptamer constructs as determined by CD spectroscopy

Aptamer construct (5 µM)	Free RNA	Complex (+2 eq. 2′-dG)	Free RNA+ 0.1 mM Mg2+	Free RNA + 5 mM Mg2+	Complex (+2 eq. 2′-dG and 0.1 mM Mg2+)
Construct 1:wild-type	57.5°C	57.9°C	58.1°C	70.9°C	58.1°C
Construct 2:P1-truncated	40.2°C/63.4°C	n.d.	42.3°C/66.3°C	79.7°C	67.8°C
Construct 3:xpt/mfl hybrid	9.8°C/63.6°C	n.d.	16.4°C/65.2°C	74.2°C	19.1°C/64.6°C