Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Apr 27;39(15):6646–6659. doi: 10.1093/nar/gkr242

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 3. — The HomeBase algorithm and database. (a) Database construction involves: (i) the identification of putative HEGs in both genomic and meta-genomic databanks using translated BLAST searches; (ii) The identification of vacant homologs using a second translated BLAST; (iii) The identification of exon boundaries and the prediction of the target based on the vacant homologs and the translation of the target to define the target range. The HomeBase database consists of a set of HEG sequences and the predicted target range of each HEase. The user may then query HomeBase with (e.g.) a human gene of interest and receive a list of HEases, which are predicted to cleave the query sequence based on a translated BLAST search against the targets in the database. (b) Statistics of the HomeBase database. (c) A pie diagram depicting the percentage of previously annotated versus newly discovered HEGs within the results of the HomeBase algorithm. (d) Classification of HomeBase HEGs into structural families.