Table 1.

Standard thermodynamic profiles for the formation of DNA duplexes at 20°C^a

ODN	NaCl	TM	ΔHcal	ΔG°20	TΔScal	ΔnNa+	Δnw
	(mM)	(°C)	(kcal/mol)	(kcal/mol)	(kcal/mol)	(mol−1 DNA)	(/mol DNA)
1	10	29.5	−80.1	−5.6	−74.5	−2.2 ± 0.2	−30 ± 4
	100	53.0	−72.3	−8.2	−64.1	−1.7 ± 0.1	−22 ± 3
2	10	23.2	−44.4	−2.3	−42.1	−1.7 ± 0.1	−15 ± 1
	100	39.2	−33.2	−3.0	−30.1	−1.2 ± 0.1	−10 ± 1
3	10	41.0	−78.2	−6.9	−71.3	−3.4 ± 0.2	−41 ± 3
	100	59.5	−92.0	−12.5	−79.5	−3.6 ± 0.2	−43 ± 4
4	10	39.4	−39.3	−3.2	−36.1	−1.0 ± 0.1	−14 ± 2
	100	51.2	−39.5	−4.6	−35.0	−0.9 ± 0.1	−13 ± 2
5	10	68.4	−31.0	−4.4	−26.6	−0.3 ± 0.1	−18 ± 2
	100	69.9	−27.7	−4.1	−23.6	−0.2 ± 0.1	−16 ± 1
6	10	56.5	−16.6	−1.8	−14.8	−0.3 ± 0.1	−7 ± 1
	100	61.0	−7.8	−1.0	−6.9	−0.1 ± 0.1	−3 ± 1

^aAll parameters are measured from UV (T_M) and DSC melting curves in 10 mM sodium phosphate buffer (pH 7.0) using ∼10 µM DNA. The observed standard deviations are: T_M (±0.7), ΔH_cal (±3), ΔG°₂₀ (±0.15), TΔS_cal (±2).