Figure 4.

Affinity-purification of Nop58-containing C/D snoRNPs from TGS1 and tgs1Δ cells. The polypeptide composition of Nop58-TAP preparations from TGS1 and tgs1Δ cells was analyzed by SDS–PAGE. The Coomassie blue-stained gel is shown in (A). The individual gel slices of the tgs1Δ lane that were subjected to proteomics analysis are demarcated on the left, along with the positions and sizes (kDa) of marker polypeptides analyzed in parallel. The identities of the relevant polypeptides in the tgs1Δ slices are indicated on the right. The numbers of peptide spectra assigned to the box C/D snoRNP core subunits and the constituents of the SSU processosome are tabulated in (B). (See Supplementary Table S4 for a fuller account of the MS analysis.).