Figure 6.

Affinity-purification of nuclear cap binding protein from TGS1 and tgs1Δ cells. The polypeptide compositions of Cbc2-TAP preparations from TGS1 and tgs1Δ cells were analyzed by SDS–PAGE. The Coomassie blue-stained gel is shown in (A). The individual gel slices subjected to proteomics analysis are demarcated on the left. The identities of the relevant polypeptides in the gel slices are indicated on the right. The CBP subunits Sto1 and Cbc2 are denoted by arrowheads. The numbers of peptide spectra assigned to CBP, karyopherin, U1, U5 and U2 snRNP components, the core Sm complex and H/ACA snoRNP components are tabulated in (B). (See Supplementary Table S6 for a fuller account of the MS analysis).