Figure 2.

(A) Different mixtures of DNA (200 ng) and Sso7c4 (0, 6, 12, 25, 50, 100, 200 and 400 ng) were run on a gel. (B) Surface Plasmon Resonance experiments showing the binding ability of Sso7c4 to duplex DNA. SPR sensorgrams for the binding of Sso7c4 to 8-mer (5′-CGCTATAGCG-3′) (left) and 16-mer (5′-CGCGTACGCGTACGCGTA-3′) (right) duplex DNA in HBS–EP buffer at 25°C. The concentration of the unbound ligand in the flow solution varies from 12.5 nM in the lowest curve to 200 nM. RU denotes response units.