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. 2011 May 5;39(15):6764–6774. doi: 10.1093/nar/gkr283

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Mapping the DNA-recognition region of Sso7c4. (A and B) overlay of ¹H-¹⁵N HSQC spectra of Sso7c4 (black) and Sso7c4/8-mer DNA complex (cyan) and Sso7c4/16-mer DNA complex (red). (C and D) combined chemical shift change (Δδ) for Sso7c4 with the addition of 8-mer DNA in (C) and 16-mer in (D). The inset figures in (C) and (D) indicate the mapping of the duplex DNA interacting with the Sso7c4 surface. (E) Ribbon diagram of Sso7c4 is shown in the same orientation as the inset surface figures in (C) and (D). (F) Hetero-nuclear NOE results with secondary structure elements color-coded as in (E).