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Sso7c4 recognizes the major groove of duplex DNA. (A) One-dimensional spectra of 12-mer DNA (5′-CAATCCGGATTG-3′) show the ring proton peaks perturbed with various concentrations of Sso7c4. (B) Duplex DNA model based on chemical-shift perturbation observed in DNA. Chemical-shift perturbations of DNA proton are proportional to the sphere radii and colored red, ΔδDNA > 0.050; orange, 0.050 ≥ ΔδDNA ≥ 0.025; and yellow, ΔδDNA ≤ 0.025.
