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Definition of the near-cognate tRNA system in S. cerevisiae. (A) Comparison of near-cognate:cognate (nc:c) ratios for individual codons as defined by the ‘third base mismatch’ rule, or by the rule proposed in ref. (20). (B) Base-pairing rules for nucleotides one and three that were used to establish near-cognate relationships. (C) The relationship between codons and decoding tRNAs in the yeast system. Cognate tRNAs are indicated in grey, near-cognate tRNAs are indicated in black.
