Table 2.
Specific activity of nuclease with free ssDNA or SSB variant/ssDNA substrates (U mg−1) |
||||||
---|---|---|---|---|---|---|
ssDNA | SSB/ssDNA | SSB113/ssDNA | SSBΔC8/ssDNA | gp32/ssDNA | gp32-SSB-Ct/ssDNA | |
ExoI | 4.7 ± 0.4a | 20.7 ± 2.9a | 5.6 ± 1.3a | 0.6 ± 0.1 | 1.0 ± 0.1 | 6.1 ± 4.6 |
SSB–ExoI | 16.3 ± 1.5 | 29.4 ± 2.1 | 27.4 ± 1.4 | 9.9 ± 1.9 | 12.4 ± 2.4 | 19.7 ± 4.0 |
aValues are from Ref. (15).