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Detection of low-abundant analytes in human blood plasma. Assays were generated for human IL-8 (A) and GDNF (B). Buffer or a blood plasma sample were spiked with either IL-8 or GDNF at concentrations between 0.1 pM and 10 nM, and measured with PEA. Signal is plotted as Ct values. Both IL-8 and GDNF, two low-abundant analytes, were detectable in plasma. Both assays showed a good sensitivity with the lower detection limit at, or below, 0.1 pM.
