Figure 3.

Effects of stargazin on glutamate and kainate desensitization in heteromeric AMPA receptors are distinct. To facilitate comparison between conditions with and without stargazin, data from Figure 1 (shown in light bars) are reproduced here. [All conditions with stargazin were significantly different from the same condition without stargazin (γ2), p < 0.05.] A, Percentage steady-state desensitization for glutamate and kainate was calculated relative to the peak response with desensitization blocked by TCM. HEK293 cells were cotransfected with stargazin and GluA1flip (1i), GluA1flip/2flip (1i/2i), GluA1flip/2flop (1i/2o), or GluA1flop/2flop (1o/2o). *Significant difference between kainate and glutamate desensitization within each receptor type, p < 0.05. ^#Significant difference relative to GluA1flip for glutamate desensitization, p < 0.01. B, Kainate efficacy was calculated relative to peak glutamate responses with desensitization for both agonists blocked by TCM. *Significant effect of stargazin, p < 0.05. ^†Significant difference from all other conditions for kainate efficacy, p < 0.01, n = 8–11 cells. C, Representative responses to glutamate and kainate in the absence (solid black line) and presence (dashed blue line) of TCM are overlaid. The blue bar indicates application of TCM and black bar agonist application.