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. 2011 Jan 3;22(8):959–968. doi: 10.1089/hum.2010.175

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Copyright 2011, Mary Ann Liebert, Inc.

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FIG. 1. — Helper-dependent adenoviral (HDAd) vectors express interleukin (IL)-10 protein in vitro. (a) Expression cassette structure. CMV, cytomegalovirus promoter from pCI plasmid; i, β-globin/IgG chimeric intron from pCI; MCS, multiple cloning site; pA, simian virus 40 poly(A) signal; 5U, 5′-untranslated region of the rabbit IL-10 gene; blocks 1, 2, 3, 4, and 5, rabbit IL-10 exons; 3U, 3′-untranslated region of the rabbit IL-10 gene. Drawing is not to scale. (b) HEK 293 cells were transduced with HDAdNull, HDAdcIL-10, or HDAdgIL-10, at the viral doses indicated (particles/ml). Conditioned medium was collected and analyzed by Western blotting for IL-10. Each lane is from cells in a separate well. Size marker is presented as kilodaltons (kDa).