FIG. 2.

Activity of vector-expressed IL-10. Rabbit peripheral blood mononuclear cells were treated with conditioned medium generated by transduction of 293 Cre4 cells with HDAdNull, HDAdgIL-10, or HDAdcIL-10. Aliquots of cells in all three groups were treated with LPS. After 5 hr, RNA was extracted and the expression of (a) TNF-α and (b) IL-1β was measured by qRT-PCR. Columns represent means of n = 3 separate wells of treated cells per group, with normal distribution assumed based on extrapolation from a larger experiment (Supplementary Fig. S2).