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. 2011 Jan 3;22(8):959–968. doi: 10.1089/hum.2010.175

FIG. 4.

FIG. 4.

Persistence of IL-10 expression and vector genomes after HDAd infusion. (a) Arteries were infused with HDAdgIL-10 (solid circles) or HDAdNull (open circles) and harvested at the indicated times. IL-10 mRNA was measured in all samples simultaneously by qRT-PCR and normalized to GAPDH mRNA in the same extracts. RNA from a single HDAdNull-infused artery harvested on day 3 served as the “calibrator” for ΔΔCt calculations (Schmittgen and Livak, 2008). Data points represent individual arteries; bars indicate group means. (b) Arteries were infused with either HDAdNull (open circles) or HDAdgIL-10 (closed circles) and harvested at the indicated times. Vector DNA was measured by qPCR and expressed as a percentage of vector DNA measured in arteries infused with the same vector and harvested on day 3 (set at 100% for both groups). Data represent means ± SEM, with n = 8–14 arteries per group. *p = 0.01; **p = 0.07 versus HDAdNull.