FIG. 7.

No evidence of systemic immunosuppression in rabbits expressing IL-10 in carotid arteries. (a and b) Liver and spleen RNA were harvested 3 days after carotid artery infusion with HDAdNull or HDAdgIL-10. IL-1β and IFN-γ mRNA were measured by qRT-PCR. Expression of IFN-γ in the liver (data not shown) was at or below the limit of detection in all samples. (c) Serum was collected from rabbits before arterial transduction with HDAdNull or HDAdgIL-10 (preoperative) and then again at harvest (14 or 28 days postoperative). Antibodies to human adenovirus type 5 were measured by ELISA, using a substrate that absorbs light at 405 nm (OD₄₀₅). Data points represent individual rabbits; bars indicate group means. *p ≥ 0.2 for comparison of these HDAdgIL-10 groups with the HDAdNull control groups at the same time point.