FIG. 1.

Analysis of phospholamban (PLB) mRNA and protein levels reveals efficacy of scAAV6-U6-shRNA-PLB in knocking down PLB expression. (A) Quantitative RT-PCR analysis demonstrates approximately 16-fold decreased PLB mRNA with short hairpin RNA (shRNA) overexpression compared with control. (B) Western blot demonstrates approximately 5-fold decreased PLB protein with shRNA overexpression compared with control. *p<0.05, n=3 per group. Tissue was collected 16–26 days postinjection. Color images available online at www.liebertonline.com/hum