FIG. 3.

AAV6 capsid does not induce a T cell-specific response in canines treated with scAAV6-U6-shRNA-PLB. Representative ELISpot data from PBMCs obtained 4 weeks postinjection and assayed for (A) interferon-γ and (B) interleukin-10 production are shown. Representative ELISpot data from lymphocytes isolated from (C) spleen and (D) heart 7 weeks postinjection and assayed for interferon-γ are shown. For all assays, cells were tested against 24 pools (5 μg/peptide, each peptide) comprising an AAV6 capsid peptide matrix, 10 μg/ml AAV6 empty capsid, phorbol 12-myristate 13-acetate (PMA)/ionomycin (positive control) or medium (negative control). The assay was performed in triplicate. Data are shown as spot-forming units/10⁶ cells. The solid line indicates 3-fold over medium background control, used to determine positive T cell responses.