FIG. 4.

IA chromatography profile (step 4 in Fig. 3). The entire bioreactor contents were applied to the preparative column. The top panel shows the full-scale UV (280 nm) absorbance profile of the flow-through and two elution peaks. Enlargements of both elution peaks are presented in the bottom panels. The particles began eluting at the onset of the pH change. The eluted fractions, 2.5 L from each load and elution, were pooled and concentrated into 0.4 L for subsequent processing. Color images available online at www.liebertonline.com/hum