FIG. 5.

Gel filtration chromatography (step 5b in Fig. 3A) was used to remove any material that coeluted from the IA chromatography step. Concentrated vector from 100-L vector production was divided into 4 × 0.1 L for gel filtration chromatography (Superdex 200). The UV (280 nm) (red curve) profile is a series of four, symmetrical, sharp peaks that contain the rAAV particles eluting in the void fractions. The four fractions were pooled for subsequent concentration and diafiltration steps. Color images available online at www.liebertonline.com/hum