FIG. 1.

A) Sperm mitochondrial membrane potential in WT, HET, and KO sperm observed by staining with the supravital fluorescent dye JC-1. Sperm were incubated at 37°C in 5% CO₂ in humidified air in TYH medium containing 5 mM glucose with (CCCP) or without (control) 10 μM of the mitochondria inhibitor CCCP. B) ATP levels in WT and KO sperm incubated 2 h with 5 mM glucose as glycolytic substrate or 1 mM pyruvate as mitochondrial substrate with (CCCP) or without (control) 10 μM of the mitochondrial inhibitor CCCP. Values are the mean ± SEM, n = 3. Letters (a, b, c) above the means denote no significant difference if identical or significant difference if not identical.