FIG. 2.
[1-13C]-glucose consumption by WT (A) and KO (B) sperm followed in real time by NMR spectroscopy. Six millimolar [1-13C]-glucose was initially added to the sperm preparation and corresponded to 100%. C) Gut and Lprod expressed in nanomole per hour and per million spermatozoa WT and KO sperm. Values for Gut and Lprod (1) were calculated from the NMR assay. Values for Lprod (2) were calculated from an enzymatic assay determining the quantity of lactate secreted by 4 × 106 spermatozoa in 1 ml TYH, 5.5 mM glucose at different times. D) Percentage of WT and KO sperm live after 22 h incubation in TYH medium containing 6 mM glucose, determined by a dye exclusion assay [16]. Values are the mean ± SEM, n = 3, *P < 0.01 compared to WT.