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. 2011 Jun 13;20(18):3578–3591. doi: 10.1093/hmg/ddr275

Figure 3.

Figure 3.

Analysis of SMN induction in adult mice. (A and B) Doxycycline was administered to the mice at 28 days of age and then western blots of (A) brain and (B) spinal cord tissue of adult mice for quantification of luciferase (luci), SMN and actin performed. Non-I indicates mice of the same genotype as induced mice but without induction, ▵7 indicates ▵7 carrier mice (SMN2+/+; Smn+/−;SMN▵7+/+) without the inducible transgene. All induced mice are heterozygous for mouse Smn (Smn+/−) and SMN is detected with a human specific antibody. (C and D) Quantification of SMN to actin ratio (white bars), and luciferase to actin ratio (gray bars), from western blots of adult mice post-induction shown in (A) and (B). (n= 3 mice for brain and n= 3 mice for spinal cord). (E) Western blot of a series of tissues harvested 10 days after SMN induction. Induction of SMN and luciferase is observed in all tissues tested. (F–H) Immunostaining of human SMN in motor neurons of spinal cord sections from (F) high-copy SMN2 mice, (G) mice induced to express SMN for 28 days and (H) non-induced mice. Immunostaining is performed with a human specific SMN antibody and animals were sacrificed at 28 days of age. Arrows indicate gems, scale bar is 50 µm.