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. 2011 Jun 13;20(18):3578–3591. doi: 10.1093/hmg/ddr275

Figure 6.

Figure 6.

Decay of SMN induction upon doxycycline removal. (A and B) Doxycycline was removed and luciferase (Luci) activity measured at 2, 3, 5 and 10 days after removal of SMN induction in (A) brain (B) spinal cord. The mice where induced for 28 days starting at PND0. (n= 3 for brain, n= 3 spinal cord). (C and D) Western blot analysis of SMN, luciferase and actin protein after removal of SMN induction in (C) brain and (D) spinal cord using a human specific monoclonal antibody. –D: days after doxycycline removal. Non-I: non-induced mice and ▵7: ▵7 carrier mice that lack the inducible transgene. (E and F) Quantification of western blots showing the luciferase to actin ratio (gray bars) and the SMN to actin ratio (white bars) and at 2, 3, 5 and 10 days after removal of doxycycline in (E) brain (n= 3 mice) and (F) spinal cord (n= 3 mice). (G and H) Real-time RT–PCR of full-length human SMN mRNA relative to cyclophilin mRNA after removal of SMN induction for 15 days (–15D, n= 5 mice) compared with 28-day induction (I, n= 8 mice) and non-induced mice (Non-I, n= 6 mice) in (G) brain and (H) spinal cord tissue.