Table 2.

A list of peptides identifying monocyte differentiation antigen CD14, detected by LC-MS/MS using three distinctive approaches methanol, methanol/PPS combination and PPS alone

MeOH
Protein name	MH+ a	Peptide	z b	Max XC c	Max ΔCN d	SC e
CD14 precursor	1972.997	R.AFPALTSLDLSDNPGLGER.G	2	2.5298	0.51	1
CD14 precursor	1777.069	R.LTVGAAQVPAQLLVGALR.V	2	3.1222	0.4924	3
CD14 precursor	1933.171	R.RLTVGAAQVPAQLLVGALR.V	2	5.3189	0.7375	2
CD14 precursor	1797.006	R.SWLAELQQWLKPGLK.V	2	3.9901	0.5849	3
MeOH/PPS
Protein name	MH+	Peptide	z	Max XC	Max ΔCN
CD14 precursor	1142.668	K.FPAIQNLALR.N	2	2.7192	0.2607	2
CD14 precursor	1972.997	R.AFPALTSLDLSDNPGLGER.G	2	5.5742	0.5339	3
CD14 precursor	1777.069	R.LTVGAAQVPAQLLVGALR.V	2	3.3594	0.5768	3
CD14 precursor	1797.006	R.SWLAELQQWLKPGLK.V	2	2.8424	0.6037	1
PPS
Protein name	MH+	Peptide	z	Max XC	Max ΔCN
CD14 precursor	2138.189	K.ITGTMPPLPLEATGLALSSLR.L	2	2.5064	0.5238	4

^aCalculated mass for a given singly-charged peptide.

^bA charge state for a given peptide.

^cMaximal Xcorr observed for a given peptide. The cross-correlation score (Xcorr) of the peptide is based on the “fit” of the MS/MS data to the theoretical distribution of ions produced for the peptide.

^dMaximal ΔCN score observed for a given peptide. The ΔCN score represents a calculated “difference” between the top two Xcorr values for the given peptide.

^eTotal spectral count for a given peptide.