Reagent	Volume (μL) (1X reaction)
DEPC treated water	10.56
2x reaction buffer	20
50% sucrose and 0.04% bromophenol blue mix	4
Forward primer SQV3F1	0.32
Reverse primer CO602	0.32
Enzyme - SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase	0.8
Total	36