Reagent	Volume (μL) (1X reaction)
DEPC treated water	13.45
60% sucrose, 0.08% cresol red mix	2
Roche HiFi system Buffer 2	2
MgCl2	0.8
dNTP	0.16
Forward primer SQV3F2	0.15
Reverse primer CD4R	0.15
Expand HiFi enzyme	0.29
Total	19