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. 2010 Dec 29;(46):2158. doi: 10.3791/2158
Dissociation Agent Dissociation Technique Cell Collection Cell Quantity Cell Purity Contamination
EDTA (3mM) without CaCl2 5, 20, 60 min. before CaCl2 addition 20, 60, 120 min. before collection - +/- +++
EDTA (6mM) without CaCl2 5, 20, 60 min. before CaCl2 addition 20, 60, 120 min. before collection +/- + +++
Trypsin-EDTA (0.5 g/L) 5, 10, 15 min. before deactivation Medium collected immediately + + ++
Collagenase II (300 U/ mL) 5, 10, 15 min. before deactivation Medium collected immediately -, +, ++ -, ++, + +
Collagenase II (600 U/ mL) 5, 10, 15 min. before deactivation Medium collected immediately +, ++, + ++ ++, ++, - -