Dissociation Agent |
Dissociation Technique |
Cell Collection |
Cell Quantity |
Cell
Purity |
Contamination |
EDTA (3mM) without CaCl2
|
5, 20, 60 min.
before CaCl2 addition |
20, 60, 120 min. before collection |
- |
+/- |
+++ |
EDTA (6mM) without CaCl2
|
5, 20, 60 min.
before CaCl2 addition |
20, 60, 120 min. before collection |
+/- |
+ |
+++ |
Trypsin-EDTA (0.5 g/L) |
5, 10, 15 min.
before deactivation |
Medium collected immediately |
+ |
+ |
++ |
Collagenase II (300 U/ mL) |
5, 10, 15 min.
before deactivation |
Medium collected immediately |
-, +, ++ |
-, ++, + |
+ |
Collagenase II (600 U/ mL) |
5, 10, 15 min.
before deactivation |
Medium collected immediately |
+, ++, +
++ |
++, ++,
- |
- |