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. 1966 Jan;91(1):442–448. doi: 10.1128/jb.91.1.442-448.1966

Comparison of Two Serologically Distinct Ribonucleic Acid Bacteriophages I. Properties of the Viral Particles

L R Overby 1,2,1, G H Barlow 1,2,1, R H Doi 1,2,2, Monique Jacob 1,2,3, S Spiegelman 1,2
PMCID: PMC315966  PMID: 5903109

Abstract

Overby, L. R. (University of Illinois, Urbana), G. H. Barlow, R. H. Doi, Monique Jacob, and S. Spiegelman. Comparison of two serologically distinct ribonucleic acid bacteriophages. I. Properties of the viral particle. J. Bacteriol. 91:442–448. 1966.—Two ribonucleic acid (RNA) coliphages, MS-2 and Qβ, have been characterized physically and serologically. MS-2 has an S20, w value of 79, a molecular weight of 3.6 × 106, a density of 1.422, and pH 3.9 as its isoelectric point. Qβ has an S20, w of 84, a molecular weight of 4.2 × 106, a density of 1.439, and an isoelectric point at pH 5.3. One host (Escherichia coli A-19) permits a distinction between the two on the basis of a marked difference in plaque size. They are distinct immunochemically, no serological cross-reaction being detectable.

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Selected References

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