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. 2010 Dec 2;(46):2066. doi: 10.3791/2066
EDAC-MES: Dissolve 50 mg/mL EDAC powder in MES Coupling Buffer
Digitonin solution (2% w/v): Dissolve digitonin powder in dH2O by heating to 95°C for 5min then cooling on ice
Lysis Buffer:  
Tris, pH 7.4 50 mM
NaCl 150 Mm
Digitonin solution 1%
Protease Inhibitors 1x
Keep on ice