EDAC-MES: | Dissolve 50 mg/mL EDAC powder in MES Coupling Buffer |
Digitonin solution (2% w/v): | Dissolve digitonin powder in dH2O by heating to 95°C for 5min then cooling on ice |
Lysis Buffer: | |
Tris, pH 7.4 | 50 mM |
NaCl | 150 Mm |
Digitonin solution | 1% |
Protease Inhibitors | 1x |
Keep on ice |