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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: J Immunol. 2011 Aug 3;187(5):2595–2601. doi: 10.4049/jimmunol.1100088

Figure 2. MPYS is required for IFN β production in response to treatment with cyclic dinucleotides.

Figure 2

A RAW264.7 lucKD or MPYSKD macrophages were treated with 9 μM c-di-AMP or 50 μM c-di-GMP and harvested for RNA at 12 hr post treatment. Quantitative PCR (TaqMan method) was performed for IFNβ gene expression and results were normalized to those of GAPDH. B. RAW264.7 lucKD or MPYSKD cells were treated with increasing concentrations of c-di-AMP or c-di-GMP as before. At 12 hr post treatment, supernatants were collected and analyzed by ELISA for IFNβ production.