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. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Exp Cell Res. 2011 Jun 22;317(16):2342–2352. doi: 10.1016/j.yexcr.2011.06.005

Figure 6. Steady-state levels and BFA-induced recruitment of GBF1 onto membranes is not affected in Cog7-deficient fibroblasts.

Figure 6

(A) Western blot analysis of GBF1 levels in WT and Cog7-deficient fibroblasts and WT and ldlB CHO cells. (B) Wild type and COG7-deficient fibroblasts were treated with BFA for 5 min. followed by disruption of the cells and cytosol/membrane fractionation. Total membrane and cytosolic pools were resolved by SDS-PAGE and GBF1 detected by Western blot analysis. (C) The percent of total GBF1 in each pool was quantified and plotted. The average from three independent experiments and the standard deviations is shown.