Table 4.
Statistical tests (F ratios) of Light-Dark (LD) cycle effects on behavioral tests a
| A. Repeated measures analysis | Between subjects | Within subjects | ||||
|---|---|---|---|---|---|---|
| Variable | dfwithin | Strain (df=7) | LD (df = 1) | Ethanol effect |
Strain x Ethanol |
LD x Ethanol |
| Day 3 Balance beam pre vs post eth. | 106 | 12.3*** | NS | 42.0*** | 2.4MS | NS |
| Day 3 Grip strength pre vs post eth. | 111 | NS | NS | 100.0*** | NS | NS |
| Day 8 ARR pre vs post ethanol | 111 | 5.1*** | NS | 7.9** | NS | NS |
| B. Between-subjects analysis (dfwithin=221) | R2 | Strain (df=7) | Ethanol (df=1) |
LD cycle (df=1) |
Strain x Eth (df=7) |
Strain x LD (df=7) |
|---|---|---|---|---|---|---|
| Body weight (with Sex in ANOVA) | 0.50 | 16.5*** | 8.4* | NS | NS | 2.7MS |
| Capture and hold score | 0.44 | 17.0*** | 8.1* | NS | NS | 2.5MS |
| Day 3 Balance beam 5 min after injection | 0.49 | 14.9*** | 81.2*** | NS | 2.4MS | NS |
| Day 3 Grip strength 7 min after injection | 0.32 | NS | 83.2*** | NS | NS | NS |
| Day 8 Grip strength 2 min after injection | 0.52 | 3.0* | 194.9*** | NS | NS | NS |
| Day 8 Open field distance 5 min after inj. | 0.63 | 33.6*** | 61.3*** | NS | 8.7*** | NS |
| Day 8 Open field rearing | 0.70 | 39.3*** | 140.7*** | NS | 9.7*** | NS |
| Day 8 ARR 30 min after injection | 0.28 | 8.0*** | 10.9** | NS | NS | NS |
NS, P > .05
MS, P <= .05
P < .01
P < .001
P < .0001. Day 2 ethanol dose was 1.2 g/kg and on Day 3 it was 1.75 g/kg.