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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: J Immunol. 2011 Jul 29;187(5):2346–2355. doi: 10.4049/jimmunol.1100534

Figure 3. TLT2 ligation potentiates neutrophil degranulation and upregulation of the activation marker CD11b in response to GPCR-mediated signaling.

Figure 3

Purified neutrophils (1×106) were incubated either with the αTLT2 mAb 1H4 (at the concentrations indicated) or biotinylated 1C5 (1μg/ml). Where indicated, streptavidin (2–40 μg) was added to mediate secondary cross-linking of TLT2. Neutrophils were stimulated with the FMLP agonist WKYMVm (W) at the indicated concentrations (A–F), the activated complement component C5a (2 ng/ml) (G), or GM-CSF (1 ng/ml) (H–I). At the indicated time points the cells were harvested and stained to detect surface expression of CD11b and analyzed by flow cytometry. Filled histograms represent CD11b expression on neutrophils incubated in medium alone. Representative histograms of CD11b expression are shown. All data are representative of at least three independent experiments.