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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: J Immunol. 2011 Jul 29;187(5):2346–2355. doi: 10.4049/jimmunol.1100534

Figure 4. TLT2 ligation results in enhanced migration of neutrophils in response to a variety of chemoattractants in vitro.

Figure 4

Purified neutrophils were subjected to migration assays using 3 μm transwell filters. Neutrophils (1×106) were placed in the upper chamber in medium alone (control) or in the presence of the indicated concentration of αTLT2 mAb. Medium alone (control) or containing chemoattractants (FMLP, 1 μM; Mip-2, 5 ng/ml; KC, 5 ng/ml; IL-8, 50–100 ng/ml or GM-CSF, 0.5–10 ng/ml) was placed in the lower chamber, as indicated. After a 1 h incubation at 37°C, the neutrophils present in the lower chamber were harvested and counted. The percentage of cells present in the lower chamber (A) and the chemotactic index (B) are shown. The chemotactic index was calculated by dividing the % migration for neutrophils incubated with each chemoattractant in the presence or absence of αTLT2 mAb by the % migration for neutrophils incubated with medium alone. Data represent the average of triplicate samples with the mean ± SD shown.