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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: J Immunol. 2011 Aug 8;187(5):2261–2267. doi: 10.4049/jimmunol.1100972

Figure 2. Intranasal challenge with insoluble components of fungal preparation induces accumulation of innate effector cells.

Figure 2

(A) Flow cytometric analysis of GFP-expressing lung eosinophils (GFP+SiglecF+SSC+) and basophils (GFP+SiglecF−DX5+) in 4get mouse lung tissue 1 day after 2 intranasal challenges with soluble or insoluble fractions from A. niger preparation (Asp). Numbers indicate the percentage of total live cells (DAPI−, not shown), and are representative of 3 independent experiments. (B) Total live cell numbers, with eosinophil and basophil subsets calculated from flow cytometry percentages as shown in (A), or percentage Gr-1+ cells (not shown). Mean±SEM, n=3/group; *p<0.01, **p<0.001, unpaired t-test.