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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: J Immunol. 2011 Aug 8;187(5):2261–2267. doi: 10.4049/jimmunol.1100972

Figure 3. Heat-sensitive proteinase activity in fungal preparation is not required for innate effector cell accumulation.

Figure 3

(A) Proteinase activity in A. niger preparation (Asp), with or without heat treatment (heat-tx), compared to trypsin control. Fluorescence produced upon cleavage of FITC-casein substrate was measured after indicated incubation times. (B) Numbers of total live eosinophils, basophils, and Gr-1+ neutrophils/monocytes in the lung 1 day after 2 intranasal Asp challenges, with or without prior heat treatment. Total live cell subset numbers were calculated from flow cytometry percentages as described in Figure 2. Mean±SEM, n=3/group; **p<0.001, significantly different from both Asp and Asp (heat-tx) groups, unpaired t-test.