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ACTH stimulation test in 15-, 30-, and 60-wk mice. ACTH stimulation test was performed by an overnight dexamethasone suppression followed by injection of ACTH as described in Materials and Methods. Blood samples were collected at 15, 30, and 60 min after ACTH injection. Corticosterone was measured by RIA, and values were plotted for 15- (A), 30- (B), and 60-wk (C) mice. Wt are represented by black squares, and Dax1−/Y are represented by white circles; *, P < 0.05, n = 5.
